Phosphatidylinositol 3-Kinase p85 Adaptor Function in T-cells
نویسندگان
چکیده
منابع مشابه
Characterization of the Met326Ile variant of phosphatidylinositol 3-kinase p85
Phosphatidylinositol 3-kinase is a key step in the metabolic actions of insulin. Two amino acid substitutions have been identified in the gene for the regulatory subunit of human p85 , Met-326Ile, and Asn-330Asp, and the former has been associated with alterations in glucose insulin homeostasis. When the four human p85 proteins were expressed in yeast, a 27% decrease occurred in the level of pr...
متن کاملRegulation of the p85/p110a Phosphatidylinositol 3*-Kinase
Our previous studies on the p85/p110a phosphatidylinositol 3-kinase showed that the p85 regulatory subunit inhibits the p110a catalytic subunit, and that phosphopeptide activation of p85/p110a dimers reflects a disinhibition of p110a (Yu, J., Zhang, Y., McIlroy, J., Rordorf-Nikolic, T., Orr, G. A., and Backer, J. M. (1998) Mol. Cell. Biol. 18, 1379–1387). We now define the domains of p85 requir...
متن کاملSrc-dependent association of Cas and p85 phosphatidylinositol 3'-kinase in v-crk-transformed cells.
Cellular changes associated with oncogenic transformation are generally caused by deregulation of signal transduction pathways. We show that, in cells transformed by the v-crk oncogene, the adapter protein Cas forms a stable complex with the p85 regulatory subunit of phosphatidylinositol 3'-kinase (PI3K) coincident with the appearance of Cas-associated PI3K activity. The interaction between Cas...
متن کاملAssociation of the p85 regulatory subunit of phosphatidylinositol 3-kinase with an essential erythropoietin receptor subdomain.
Using an active, HAI epitope-tagged form of the murine erythropoietin (EPO) receptor and via direct coimmunoprecipitation, the p85 regulatory subunit of phosphatidyl inositol-3 kinase (p85/PI3-K) is shown to associate with the EPO receptor in transfected FDC-P1 cell lines. Coimmunoprecipitation of p85 with epitope-tagged EPO receptors was observed initially in FDC-HER cells labeled metabolicall...
متن کاملTyrosine phosphorylation of p85 relieves its inhibitory activity on phosphatidylinositol 3-kinase.
Under resting conditions, the p85 regulatory subunit of phosphatidylinositol 3-kinase (PI3K) serves to both stabilize and inactivate the p110 catalytic subunit. The inhibitory activity of p85 is relieved by occupancy of the NH(2)-terminal SH2 domain of p85 by phosphorylated tyrosine. Src family kinases phosphorylate tyrosine 688 in p85, a process that we have shown to be reversed by the activit...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2002
ISSN: 0021-9258
DOI: 10.1074/jbc.m107648200